symphytum officinale homeopathic remedy uses

Interestingly, gene expression analysis for Runx-2 (n = 10), Osteopontin (n = 10), Osteocalcin (n = 10) and alkaline phosphatase activity (n = 8) also showed increased osteogenesis with the addition of Symphytum officinale to BDM, specially mother tincture. The efficiency of osteogenic differentiation (2 weeks) for different doses of Symphytum officinale was determined by flow cytometry (n = 10) for osteoblast marker, Osteocalcin, and most doses of Symphytum officinale enhanced osteogenesis. MTT assay showed that the selected doses of SO did not induce any cytotoxicity in MSCs (24 h). Gene expression analyses for osteoblast markers (Runx-2, Osteopontin and Osteocalcin) were evaluated in differentiated osteoblasts using Q-PCR.įlow cytometry (CD90, CD105) detected MSCs isolated from bone marrow (93-98%). Different homoeopathic doses of SO (MT, 3C, 6C, 12C and 30C) were added to the basic differentiation medium (BDM) and efficiency of MSCs differentiating into osteoblasts were measured by evaluating expression of Osteocalcin using flow cytometry, and alkaline phosphatase activity using ELISA. Osteogenic differentiation was induced in MSCs with β-glycerophosphate, ascorbic acid and dexamethasone over 2 weeks. Cytotoxicity of SO was evaluated by MTT assay. MSCs were isolated, expanded and characterized by flow cytometry (CD90, CD105). This study aimed to determine if homoeopathic doses of SO could augment osteogenesis in MSCs as they differentiate into osteoblasts in vitro.īone marrow samples were obtained from patients who underwent bone grafting procedures (n = 15).

Symphytum officinale (SO) is a medicinal plant and in homoeopathic literature, believed to accelerate bone healing.

Mesenchymal stem cells (MSCs) are multipotent stem cells possessing regenerative potential. She was admitted in hospital via Accident & Emergency department after a series of plain radiographs confirmed a closed displaced oblique fracture of the tibia and fibula (Figs.1a & 1b). She managed to call the emergency number and was taken to local hospital by ambulance. She had suffered an acute trauma to her lower third of the left leg with extensive soft tissue contusion, haematoma which extended down into her foot with swelling and pain. She does not recall how or, what happened and has poor recollection of the mechanism of injury. The current injury occurred when she fell off her bed in the middle of the night with excruciating pain in her leg. She has not had any previous history of fracture or any other injury. She lives on her own, a non-smoker, partakes in social alcohol and keeps good general health with no past or present medical, surgical or drug history. Case Study A female aged 62 years works full time as a Ceremony Officer and keeps fit by walking but admits that she is not an active person.

Symphytum officinale homeopathic remedy uses